![](https://parts.igem.org/images/partbypart/icon_dna.png)
DNA
Part:BBa_K4373003
Designed by: Mandy Hobusch, Leon Eisentraut Group: iGEM22_Lund (2022-09-24)
C-P-Lyase encoding sequence
Two pathways for the degradation of glyphosate have been described - the sacrosine pathway and the aminomethylphosphonic acid (AMPA) pathway. In the former reaction, an enzyme called C-P-Lyase catalyses the cleavage of C-P bonds from glyphosate forming sarcosine and organophosphate [1]. Many bacteria and fungi found in enviromental samples such as soil have been reported to perform this form of glyphosate degradation pathway [2]. For our project, the multimeric enzyme domain, so called CP-lyase was isolated from NCBI gene bank [3].
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 1627
Illegal EcoRI site found at 1969
Illegal PstI site found at 1654
Illegal PstI site found at 3061
Illegal PstI site found at 3360
Illegal PstI site found at 3833 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1627
Illegal EcoRI site found at 1969
Illegal PstI site found at 1654
Illegal PstI site found at 3061
Illegal PstI site found at 3360
Illegal PstI site found at 3833 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1627
Illegal EcoRI site found at 1969
Illegal BglII site found at 837
Illegal BglII site found at 1605
Illegal BamHI site found at 1461
Illegal XhoI site found at 1056 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 1627
Illegal EcoRI site found at 1969
Illegal PstI site found at 1654
Illegal PstI site found at 3061
Illegal PstI site found at 3360
Illegal PstI site found at 3833 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 1627
Illegal EcoRI site found at 1969
Illegal PstI site found at 1654
Illegal PstI site found at 3061
Illegal PstI site found at 3360
Illegal PstI site found at 3833
Illegal NgoMIV site found at 2143
Illegal NgoMIV site found at 3018
Illegal NgoMIV site found at 3458
Illegal AgeI site found at 662
Illegal AgeI site found at 1121
Illegal AgeI site found at 1497 - 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
biology | |
protein |